Addition Of Gibberellin (GA3) to Gladiolus Cormel Growth (Gladiolus communis L.) on Various Medium in Vitro
Abstract
Gladiolus flower can be multiplied by using corm or cormel. The multiplication of this flower using plating material takes a long time, about 3,5-6 months. By using in vitro method, the seed of this flower can be produce in relatively short time. The aim of this research was to determine the interaction of gibberellins on any kind of media (MS, B5, and MS + vitamin B5), to determine the perfect combination of concentration of Gibberelin, and to determine the perfect type of media that can be use for induction of root and shoot of gladiolus cormel. The research design used was a Completely Randomized Design (CRD) of the factorial pattern with 3 replications. The first factors (concentration of Gibberellin) G1: 1 ppm, G2: 2 ppm, dan G3: 3 ppm, the second factors (type of media) M1: MS , M2: B5 (Gamborg) , M3: MS + B5 vitamin. The result showed that the addition of various concentration of Gibberelin (GA3) and any type of media had no significantly improve the growth of gladiol cormel explant, by addition concentration of Gibberelin 2 ppm has significantly improved fresh weight of planlet and percentage of life, by addition B5 media, has significantly improved length of root and growth of shoot, and by using MS media with B5 vitamin has significantly improved the growth of shoot, height and fresh weight of planlet.
Keywords: Gladiol, gibberellin, plant media, in vitro
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Agrawal, K. C. 1989. Physiology and Biochemistry of Respiration. Agro Botanical Publisher. New Delhi. 187 p.
BPS. 2014. Statistik Tanaman Hias (Statistics of Ornamental Plants). Badan Pusat Statistik. Jakarta. Hal 3 – 10.
Dewi, I., SJ. Harjosudarmo dan RG. Birch. 1997. The Effect of 2,4-D, NAA and Picloram on Somatic Embrio Genesys and Plant Regeneration from Immature Peanut Seed. Jurnal Biotek Pertanian. Vol 2 (1): 23 – 30.
Ellya, 2009. Pangan, Gizi, Teknologi dan Konsumen. Penerbit Gramedia. Jakarta.
Gardner, FP., RB. Pearch and RL. Mitchel.1991. Physiology of Crop Plants. The Iowa State University Press. 428 p.
Handayani, E, S. Samsudin dan Basri. 2013. Pertumbuhan Eksplan Buah Naga pada Posisi Tanam dan Komposisi Media yang Berbeda Secara In Vitro. Jurnal Agrotekbis 1 (1): 1 – 7, April 2013.
Hendaryono, D.P.S dan A. Wijayani. 2013. Teknik Kultur Jaringan. Kanisius. Yogyakarta.
Herlina, D. dan B. Haryanto. 2005. Perbanyakan Gladiol dalam Gladiol. Balai Penelitian Tanaman Hias. Jakarta. Hal 21–33.
Komatsuda, T., W. Zee dan S. Oka. 1992. Maturation and Germination of Somatic Embryos as Affceted by Sucrose and Plant Growth Regulations in Soybean (Glycine gracilis Skvortz and Glycine max L Mers). Plant Cell Tissue and Organ Culture. Vol 28: 103 – 113
Kusumawati, E., Sari, P.Y dan Purnaningsih, T. 2015. Pengaruh NAA dan BAP terhadap Inisiasi Tunas Mengkudu (Morinda citrifolia) Secara In Vitro. Jurnal Agrisains Budidaya Tanaman Perkebunan. Vol 1(1): 8 – 17
Murniati dan E. Zuhry. 2002. Peranan Gibberelin terhadap Perkecambahan Benih Kopi Robusta tanpa Kulit. Jurnal Sagu. Vol 1 (1): 1-5
Rukmana, R. 2005. Gladiol. Kanisius. Yogyakarta
Santosa, U dan F. Nursandi. 2001. Kultur Jaringan Tanaman. Penerbit UMM. Malang.
Setiawati, T., Titin, S., Nia, R. 2006. Pengatur Zat Pengatur Tumbuh terhadap Pertumbuhan Gladiol (Gladiolus x gandavensis) dan Uji Resistensinya Terhadap Ekstrak Jamur Fusarium oxysporum Secara In vitro. Jurnal Biotika. Vol 5(2): (7-12).
Srilestari, R. 2005. Induksi Embrio Somatik Kacang Tanah Pada Berbagai Macam Vitamin dan Sukrosa. Jurnal Ilmu Pertanian. Vol 12(1): 43 – 50.
Sutater, T. 2001. Pengaruh Pembelahan Subang dan Pemupukan K terhadap Pertumbuhan dan Produksi Gladiol Kultivar Salem. Buletin Penelitian Hortikultura. Vol 25(1): 107 – 1
DOI: https://doi.org/10.31315/agrivet.v23i2.4811
DOI (PDF): https://doi.org/10.31315/agrivet.v23i2.4811.g3477
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