Production, Characterisation, and Isolation of Lipase from Aspergillus niger by using Palm Oil as Inducer

Authors

  • Sri Wahyu Murni Program Studi Teknik Kimia, Fakultas Teknologi Industri, UPN “Veteran” Yogyakarta Jl. SWK 104 (Lingkar Utara), Condong Catur, Yogyakarta 55283
  • Siti Diyar Kholisoh Program Studi Teknik Kimia, Fakultas Teknologi Industri, UPN “Veteran” Yogyakarta Jl. SWK 104 (Lingkar Utara), Condong Catur, Yogyakarta 55283
  • Tanti D.L. Program Studi Teknik Kimia, Fakultas Teknologi Industri, UPN “Veteran” Yogyakarta Jl. SWK 104 (Lingkar Utara), Condong Catur, Yogyakarta 55283
  • Petrissia E.M. Program Studi Teknik Kimia, Fakultas Teknologi Industri, UPN “Veteran” Yogyakarta Jl. SWK 104 (Lingkar Utara), Condong Catur, Yogyakarta 55283

DOI:

https://doi.org/10.31315/e.v12i1.1034

Keywords:

Aspergillus niger, enzyme activity, lipase, palm oil

Abstract

The objective of the research was to produce, isolate and characterise lipase from Aspergillus niger, and therefore inducted it by using palm oil. The lipase enzyme was produced through a batch fermentation process in a 1.4 liters-fermentor. Fermentation was carried out at room temperature, initial pH of 7, the stirring speed of 250 rpm, aeration rate of 1 vvm, and inducer concentration of 3%-m/v palm oil/ml. Enzymes was characterised at several temperature and pH variations. The lipase showed the optimum performance at pH of 7 and temperature of 30 °C with the activity of 1.5 U / ml. Isolation of lipase yielded a 4-times-increase in its activity by using 90% ammonium sulfate.

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Published

2015-07-09

How to Cite

Murni, S. W., Kholisoh, S. D., D.L., T., & E.M., P. (2015). Production, Characterisation, and Isolation of Lipase from Aspergillus niger by using Palm Oil as Inducer. Eksergi, 12(1), 01–04. https://doi.org/10.31315/e.v12i1.1034

Issue

Vol. 12 No. 1 (2015)

Section

Artikel

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