Penghambatan Browning pada Ekstrak Apel Malang dengan Asam Organik
Keywords:
apples, browning, enzymatic browning, antibrowning, spectrophotometerAbstract
Browning of raw fruits is a major problem in the food processing industry, causing quality loss, decreased market value, deleterious changes in the appearance and organoleptic properties of the food product. Browning reactions could happen due to enzymatic and non-enzymatic reactions (maillard reactions). Enzymatic browning reactions occur on the substrate containing phenol groups, react with polyohenoloxidase enzyme (PPO) producing melanin compounds which contain brown pigments. Various methods could be used to prevent browning such as blanching, pH adjustment, and addition of anti-browning agents. The effectiveness of citric acid, ascorbic acid, and lemon as a browning inhibitor were studied. The anti-browning agents were carried out on varied concentration (0.1%; 0.3%; 0.5%; 1.0%; and 2.0 %). Apple PPO activities were determined by measuring the absorbance at 422 nm with a spectrophotometer. Addition of lemon 2.0% becomes the best inhibitor to prevent browning, obtaining % inhibition about 83.30%. The results of analysis of variance (ANOVA) were further processed using least square difference’s method, showed significant results for the effect of all type variations of anti-browning agents.
References
Ioannou M. Prevention of enzymatic browning in fruit and vegetables. European Scientific Journal. 2013; 9 (30): 310-341.
Janovitz-Klapp A. Inhibition studies on apple polyphenol oxidase. J. Agric. Food Chem. 1990; 38 (4): 926-931.
Kaushal B. B. L. Preparation and evaluation of some value-added products from hill lemon (Citrus pseudolimon) fruits. Indian Journal of Agricultural Sciences. 2001; 71 (11): 691-694 .
Lindley M. The Impact of food processing on antioxidants in vegetable oils, fruits and vegetable. Trends in Food Science & Technology. 1998; 9: 336-340.
McEvily A. Inhibition of enzymatic browning in foods and beverages. Critical Review in Food Science and Nutrition. 1992; 32 (3): 253-273.
Mishra B. B. Polyphenol oxidases: biochemical and molecular characterization. Enzyme Engineering. 2006; 5 (1): 1-9.
Ozoglu H. Inhibition of enzymic browning in cloudy apple juice with selected antibrowning agent. Food Control. 2002; 13 : 213-221.
Sappers G., Miller R. Enzymatic browning control in potato with ascorbic acid-2-phosphates. Journal of Food Science. 1992; 57 (5): 1132-1135.
Downloads
Published
Issue
Section
License
Syarat yang harus dipenuhi oleh Penulis sebagai berikut:- Penulis menyimpan hak cipta dan memberikan jurnal hak penerbitan pertama naskah secara simultan dengan lisensi di bawah Creative Commons Attribution License yang mengizinkan orang lain untuk berbagi pekerjaan dengan sebuah pernyataan kepenulisan pekerjaan dan penerbitan awal di jurnal ini.
- Penulis bisa memasukkan ke dalam penyusunan kontraktual tambahan terpisah untuk distribusi non ekslusif versi kaya terbitan jurnal (contoh: mempostingnya ke repositori institusional atau menerbitkannya dalam sebuah buku), dengan pengakuan penerbitan awalnya di jurnal ini.
- Penulis diizinkan dan didorong untuk mem-posting karya mereka online (contoh: di repositori institusional atau di website mereka) sebelum dan selama proses penyerahan, karena dapat mengarahkan ke pertukaran produktif, seperti halnya sitiran yang lebih awal dan lebih hebat dari karya yang diterbitkan. (Lihat Efek Akses Terbuka).