Production of Bioethanol from Solid Wastes of Tapioca Flour Industry Through Enzymation and Fermentation Process

Abstract

Solid wastes from the tapioca flour industry still contain a lot of starch which is only used as animal feed. Even though the starch content in the solid waste can be converted into bioethanol to increase the benefits of solid waste itself. The purpose of this study was to utilize the potential of solid waste from the tapioca flour industry to produce bioethanol through an enzymatic and fermentation process. The solid waste was firstly dried in an oven at 60⁰C for 24 hours, then it was mashed. The fine size of solid waste was then conducted a liquefaction process with the addition of water to a volume of 2 litres, then was added CaCl₂ 40 ppm and α-amylase of 2-5% (w/v) and heated at 90⁰C-100⁰C for 2 hours. The next step was that saccharification process was conducted, which was a solution added with HCl to pH 4, and glucoamylase of 2-5% (w/v) then was heated at a temperature of 60⁰C for 4 hours. The result showed that the highest reducing sugar obtained was 100 g/l with the use of solid waste of 75 g/l.