Pengaruh Konsentrasi Asam Klorida (HCl) Pada Hidrolisis dan Waktu Fermentasi Terhadap Limbah Padat Sagu Menjadi Bioetanol

Abstract

Bioethanol is a bioenergy that can be used as an alternative fuel. One of the raw materials that can be utilized is sago solid waste. Sago pulp biomass, which is a lignocellulose material, contains considerable potential as a basis for bioethanol based on the availability of certain components (starch and cellulose) it contains. Cellulose content found in sago solid waste can be converted into bioethanol through hydrolysis and fermentation processes. This study aims to synthesize bioethanol from sago solid waste, determine the effect of HCl concentrations on the hydrolysis process and determine the optimum time of bioethanol production from sago pulp waste by the method of separate hydrolysis and fermentation (SHF). The research began with pretreatment of sago pulp using 1M NaOH, then the hydrolysis process used HCl with a variation of 1 M, 2 M and 3 M at 100^oC for 3 hours. Then the hydrolysis results are fermented using Saccharomyces cerevisiae. The fermentation process lasts for 24 hours, 48 hours, 72 hours, 96 hours and 120 hours. The results showed that the largest sugar concentration obtained at the 2 M HCl hydrolysis was 140.18 gr / L and the best bioethanol content was obtained from fermentation for 96 hours is 7% or 55.25 gr / L.