MORPHOLOGICAL AND MORPHOMETRIC IDENTIFICATION OF ENTOMOPATHOGENIC NEMATODES IN CORN FIELDS IN GUNUNGKIDUL REGENCY
DOI:
https://doi.org/10.31315/agrivet.v31i3.15952Keywords:
morphology, morphometry, population, Tenebrio molitor, white head trayAbstract
Corn productivity often declines due topest attacks, but can be controlled through the use of biological agents such asentomopathogenic nematodes (EPN). This study aims to explore andidentify EPN in corn fields in Gunungkidul Regency morphologically and morphometrically. Soil samples were taken in five districts, namely Wonosari, Playen, Patuk, Karangmojo, and Semanu. NEP trapping was carried out using Tenebrio molitor larvae, then isolatedusing the white head tray method and observed morphologically and morphometrically using a microscope. The results showed that the highest NEP population NEP population was found in Playen Subdistrict (462.67 NEP/5 ml) and the lowest in Patuk Subdistrict (7.33 NEP/5 ml). Population variation is thought to be influenced by differences in soil texture and cultivation systems. Based on the morphological and morphometric characteristics of the NEP found in Gunungkidul corn fields, they were identifiedas Heterorhabditis indica, with a body length ranging from 438.77–501.9 µm. These findings indicate the potential of H. indica as a biological agentin pest control in corn farming ecosystems in the Gunungkidul.
Keywords : morphology, morphometry, population, Tenebrio molitor, white head tray
References
Abolafia, J., & Peña-Santiago, R. 2019. Description of Metarhabditis giennensis sp. n. (Nematoda, Rhabditida, Rhabditidae) from decaying wood of a river-bank forest in the southern Iberian Peninsula. Zootaxa, 4652(1).
Ali, M., Allouf, N., & Ahmad, M. 2022. First Report of Entomopathogenic Nematode Steinernema affine (Nematoda: Steinernematidae) in Syria and its Virulence Against Galleria Mellonella L.(Lepidoptera: Pyralidae). Egyptian Journal of Biological Pest Control, 32(1), 101.
Asif, M., Prasad, J. S., Khan, R., Somasekhar, N., & Tahseen, Q. 2013. A revision of the genus Metarhabditis (Nematoda: Rhabditidae) with description of three known species, a key to the identification of congeners and discussion of their relations. Journal of Natural History, 47(31–32), 2599–2622.
Badan Pusat Statistik 2025. Analisis Produktivitas Jagung dan Kedelai di Indonesia, 2024 (Hasil Survei Ubinan). https://www.bps.go.id/id/publication/2025/10/02/561a7fb4534ef4201b447f5b/analisis-produktivitas-jagung-dan-kedelai-di-indonesia--2024--hasil-survei-ubinan-.html
Erdiansyah, I., & Fauziah, A. N. 2024. Eksplorasi, Identifikasi, dan Karakterisasi Nematoda Entomopatogen pada Lahan Jagung dan Padi. Jurnal Ilmu Pertanian Indonesia, 29(2), 173-178.
Gee, G. W., & Bauder, J. W. 1986. Particle‐Size Analysis. Methods of soil analysis: Part 1 Physical and mineralogical methods, 5, 383-411.
Jaffuel, G., Blanco-Pérez, R., Hug, A.S., Chiriboga, X., Meuli, R.G., Mascher, F., Turlings, T.C. and Campos-Herrera, R., 2018. The evaluation of entomopathogenic nematode soil food web assemblages across Switzerland reveals major differences among agricultural, grassland and forest ecosystems. Agriculture, Ecosystems & Environment, 262, pp.48-57.
Malan, A.P., Knoetze, R. & Tiedt, L.R. 2014. Heterorhabditis noenieputensis n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa. Journal of Helminthology, 88(2): 139-151.
Nguyen, K. B., & Hunt, D. J. 2007. Entomopathogenic nematodes: Systematics, phylogeny and bacterial symbionts. Nematology Monographs and Perspectives, 5, 1–816. Brill Academic Publishers.
Nguyen, K. B., Tesfamariam, M., Gozel, U., Gaugler, R., & Adams, B. J. 2006. Steinernema abbasi n. sp. (Rhabditida: Steinernematidae) from the Sultanate of Oman. Nematology, 8(2), 319–332.
Prabowo, H. 2012. Pemanfaatan nematoda patogen Steinernema spp. isolat Malang dan Nusa Tenggara Barat dalam pengendalian Spodoptera litura L. yang ramah lingkungan. Bumi Lestari (Jurnal), 12(2), 350–356.
Sucipto. 2009. Nematoda entomopatogen Heterorhabditis isolat lokal Madura sebagai pengendalian hayati hama penting tanaman hortikultura yang ramah pada lingkungan. Agrovigor: Jurnal Agroekoteknologi, 2(1).
Susurluk, A., & Ehlers, R. U. 2008. Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. Biocontrol Science and Technology, 18(9), 987–996.
Tarasco, E., Fanelli, E., Salvemini, C., El-Khoury, Y., Troccoli, A., Vovlas, A., & De Luca, F. 2023. Entomopathogenic nematodes and their symbiotic bacteria: from genes to field uses. Frontiers in Insect Science, 3, Article 1195254.
