CALLUS REGENERATION OF Chrysanthemum AFTER GAMMA RAY IRRADIATION FOR THE RESILIENCE OF MEDIUM PLAIN

Abstract

Development of chrysanthemum plants in the medium plains is still facing obstacles due to
unfavorable climate. Until today, the availability of tolerant chrysanthemum seeds grown in the medium
plains is not maximized so that the necessary effort in order to increase the genetic diversity as the
material selection to obtain tolerant chrysanthemums grown in medium plain. This research is being
conducted in order to follow up the problems of availability of tolerant chrysanthemums seeds grown in
the medium plains at several stages. Stage one is the induction of callus after gamma ray irradiation in
vitro. This research has been conducted in tissue culture laboratory of the Faculty of Agriculture UPN
"Veteran" Yogyakarta from February to June 2015. Tested regeneration media is ½ MS with the addition
of IAA 0.1 mg/l; 0.2 mg/l; 0.3 mg/l; 0.4 mg/l and 0.5 mg/l. The results showed that ½MS regeneration
media with the addition of IAA 0.3 mg/l spur sprouts emerge percentage (100%); while growing sprouts
(8.67 days), height are about sprouts (3:10 cm) and number of sprout (7:00). Regeneration medium
Treatment with kinetin 2 mg/l + IAA 0.5 mg/l gives the number of root growth (13) and root length (4:07
cm)
Keywords: Chrysanthemum, tolerant medium plains, in vitro selection, gamma ray irradiation,